The Hellenic Republic is one of the most important partners of Ukraine in the region of the South Balkans and the Eastern Mediterranean. In this respect, an important area of cooperation between Ukraine and Greece is the development of relations between sister-cities and regions, which is based on the agreement between the two countries and a number of legal documents on interregional cooperation. Nowadays signed more than 25 such agreements have been signed between different cities and regions of Ukraine and Greece, including twinning agreements between the cities of Kyiv and Athens, Odessa and Piraeus, the city Mariupol- island Rhodes, urban area Sartana of the city of Mariupol -Zografou, urban area Sartana of the city of Mariupol–island Kithnos, the city of Kylia of the Odessa region – the city Farsala, and others.

Taking into account the importance of the economic component of regional cooperation between Ukraine and Greece, the Agreement on cooperation between Zhytomyr Chamber of Commerce and the Hellenic-Ukrainian CCI, the Memorandum of Cooperation between the Ukrainian Chamber of Commerce and Industry and the Federation of Greek Industry, the Agreement on Cooperation between Khmelnytsky CCI and the Piraeus Chamber of Commerce.